Objective To establish a simple,rapid and accurate colloidal gold test strip for the detection of P.gingivalis in human saliva.Methods The sandwich ELISA system was established by using monoclonal antibodies E10 and G3 recoginizing different epitope of P.gingivalis.The specificity and sensitivity of sandwich ELISA in the recognition of P.gingivalis were identified.Colloidal gold test strips were prepared with monoclonal antibodies E10 and G3 to detect P.gingivalis.Colloidal gold test strip and real-time quantitative PCR were used to detect P.gingivalis in the saliva of the subjects with periodontitis.The difference between the two methods in detecting P.gingivalis was analysed.Results The sandwich ELISA system established by monoclonal antibodies E10 and G3 could specifically recognize P.gingivalis,and the sensitivity for recognition of P.gingivalis was more than 5×107 CFU/mL.The colloidal gold test strip prepared by monoclonal antibodies E10 and G3 specifically recognized P.gingivalis.The colloidal gold test strip and PCR showed no significant difference in the detection of periodontitis.The positive detection rate of collidal gold test strip was 40%in periodontitis group and 10%in healthy group.Conclusions The colloidal gold test strip could specifically detect P.gingivalis and had the potential to detect P.gingivalis in saliva.
关键词
胶体金试纸条/牙周炎/牙龈卟啉单胞菌/单克隆抗体/血凝素2
Key words
Colloidal gold test strip/Periodontitis/Porphyromonas gingivalis/Monoclonal antibody/rHA2
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