Study on the Detection Method of Illegal Dyes Sudan IV and Scarlet 808 in Xiaoshuan Zaizao Pills
The aim is to establish a detection method for illegal dyes Sudan IV and Scarlet 808 in Xiaoshuan Zaizao Pills.High-performance liquid chromatography(HPLC)was used for the quantitative determination of the two dyes in Xianshuan Zaizao Pills.The Phenomenex Luna C18 column(250 mm×4.6 mm,5 μm)was employed with an acetonitrile-0.02 mol/L ammonium acetate solution as the mobile phase for gradient elution.The flow rate was set at 1.0 mL/min,the column temperature at 40℃,and the detection wavelength at 520 nm.Results show that the linear ranges of Sudan IV and Scarlet 808 were 0.001 48～0.029 60 μg and 0.002 50～0.050 00 μg,respectively,the average recovery(n=6)was 101.68%and 101.64%,and RSD was 0.53%and 0.75%.It can be concluded that the detection method is convenient and accurate,suitable for the determination of the illegal dyes Sudan IV and Scarlet 808 in Xiaoshuan Zaizao Pills.
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