Study on the Absorption Distribution and Translocation Trends of Arsenic in Lonicera Japonica Thunb Using Synchrotron Radiation μ-XRF
Environmental factors significantly impact the accumulation of arsenic in Lonicera japonica Thunb.This thesis employed a hydroponic method to simulate stress from organic and inorganic arsenic and investigate the changes in key enzyme activities in Lonicera japonica Thunb.Furthermore,synchrotron radiation X-ray fluorescence spectroscopy(μ-XRF)was used to analyze the absorption,distribution,and translocation trends of arsenic within the plant.The Lonicera japonica Thunb plants were subjected to arsenic stress at different concentrations and forms,and biochemical indicators of their leaves,including peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD),were measured.The biochemical results showed that under low-concentration arsenic stress,multiple biochemical indices could respond positively.The effects of low-concentration inorganic arsenic and high-concentration organic arsenic stress on POD activity were similar,with prolonged exposure to both forms of arsenic significantly increasing POD activity.High-concentration organic arsenic stress caused a sharp decline in CAT activity,indicating damage to the plant's protective mechanisms.Changes in SOD activity varied depending on the form of arsenic,with a V-shaped fluctuation under inorganic arsenic conditions and a continuous decrease under organic arsenic conditions,indicating limited cellular regulatory capacity in plants.μ-XRF analysis revealed that inorganic arsenic primarily accumulates in the epidermal cortex of the roots,with the roots and stems forming an interception barrier.In contrast,organic arsenic concentrates in the root center and is mainly distributed in the outer epidermis of the stem.These findings provide scientific evidence for assessing the impact of heavy metal contamination on the quality of medicinal plants and exploring strategies to reduce heavy metal accumulation in medicinal plant parts.
arsenicLonicera japonica Thunbantioxidant enzymesmorphological distribution
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