Immune-Binding Activity of Scylla paramamosain Matrix Reduced by Boiling Processing
Allergen was the main reason for crustacean allergy,processing could effectively reduce the allergenicity of allergens,while the research based on food matrix needed to be further studied in-depth.A biomimetic evaluation digestion and transport system was constructed based on the simulated continuous digestion of the gastrointestinal phase and the human colon Caco-2 cell transport model.The digestibility of the crab matrix and the transport of digestive products after boiling processed were systematically evaluated based on the crab matrix.Based on the specific binding of antigens and antibodies,the effect of boiling processing on the immune-binding activity of boiled crab matrix and digestive transport products was clarified.The main reasons for the changes in the immune-binding activity of crab matrix and digestive transport products were further analyzed from the structure perspective.The results showed that the digestibility of the boiled crab matrix was increased,it was easier to pass through the Caco-2 cell barrier.The immunoglobulin(Ig)E binding activity of crab matrix and biomimetic digestive transport products were reduced by 38.85％±0.94％and 14.82％±0.24％,respectively.The IgG binding activity of the heat stable allergens tropomyosin and sarcoplasmic calcium binding protein in matrix was significantly decreased.It was mainly due to the boiling process changed the fiber bundle arrangement,improved the digestibility of the crab matrix,and lowered the immune-binding activity.Changes in the structure of protein components in the matrix reduced the immune-binding activity of biomimetic digestive transport products and heat stable allergens.Basing on the food matrix,this study analyzed the effect of boiling on the immune-binding activity of crab matrix,improved the study on the effect of processing on the immune-binding activity of aquatic products,and provided a theoretical foundation for the exploration and evaluation of hypoallergenic aquatic product processing methods.
万方数据
维普
