To investigate the anti-atherosclerosis effect and mechanism of 5-heptadecylresorcinol(AR-C17),a bioactive substance in whole grain foods,human umbilical vein endothelial cells(HUVEC)were induced with trimethylamine-N-oxide(TMAO)to establish adamage model.The protective effect of AR-C17 on HUVEC and its mechanism were investigated by detecting endothlial cell migration,mitochondrial function,apoptosis level and related protein expression.The results showed that 0.5,1.0 and 2.0 μmol/L AR-C17 could significantly inhibit TMAO-induced cell death,and the cell viability of HUVEC were increased to 69％,71％and 72％,respectively.Three dosages of AR-C17 also significantly improved the endothelial function of HUVEC,and the cell migration rates were increased to 47％,55％and 71％.The result of mitochondrial function showed that AR-C17 could significantly reduce the level of intracellular and mitochondrial reactive oxygen species induced by TMAO and the disorder of mitochondrial membrane potential.AR-C17 significantly increased the basal respiration,ATP production and maximum respiratory oxygen consumption rates,indicating that AR-C1 7 significantly alleviated the TMAO-induced mitochondrial dysfunction.Meanwhile,the results showed that AR-C17 reduced the TMAO-induced apoptosis rate of HUVEC from 32％to 19％.The expression levels of proteins related to the mitochondria-dependent apoptotic pathway indicated that AR-C17 significantly reduced the ratio of B-cell lymphoma-2(BCL-2)-associated X protein and BCL-2 expression,while inhibiting cytochrome C expression in the cytoplasm and increasing its expression level in mitochondria.The results showed that AR-C17 alleviated TMAO-induced HUVEC injury by improving mitochondrial function and inhibiting mitochondria-dependent apoptosis.The aim of this study was to provide the theoretical basis for development and utilization of whole grain functional foods against cardiovascular.
5-heptadecylresorcinolendothelial celltrimethylamine-N-oxideapoptosismitochondrial function
维普
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