White-tailed sea eagle(Haliaeetus albicilla),a large raptor belonging to Accipitridae of Accipitriformes,is a first-class national protected animal in China.This top predator plays a significant role in maintaining ecosystem balance and biodiversity.During the winter,a large wintering population is present in Jingxin Wetland in Hunchun city,Jilin province.To study the population genetics of white-tailed sea eagles,it is necessary to develop microsatellite SSR(simple sequence repeat)and sex identification primers.In this study,microsatellite and sex identification primers were designed based on the whole genome data of white-tailed sea eagle(NCBI),and verified with white-tailed sea eagle fecal samples collected from Jingxin Wetland.A total of 9 pairs of SSR primers and 2 pairs of sex identification primers were confirmed,which will be useful for future studies of the white-tailed sea eagle.
关键词
SSR分子标记/性别鉴定/引物开发/粪便样品
Key words
SSR molecular marker/sex identification/primer exploitation/fecal samples
维普
万方数据