The elastic modulus changes of trabecular meshwork in rats under high intraocular pressure
Objective To investigate the changes in the elastic modulus of the trabecular meshwork tissue in rats under sustained high intraocular pressure at different time intervals. This study aims to provide a foundation for a deeper understanding of the mechanical properties of the trabecular meshwork tissue and its relationship with aqueous humor outflow resistance. Methods Eighteen male SD rats weighing between 260-300 g were selected. The experimental group underwent elevated intraocular pressure by cauterizing the superior venous angle in the right eye,while the left eye served as the control without any treatment. Eyes were enucleated at 2 weeks,3 weeks,and 4 weeks for frozen cross-sectional slices of the trabecular meshwork tissue. Nanoindentation experiments were conducted using atomic force microscopy in the trabecular meshwork region. The indentation curves were fitted using the Sneddon contact model to determine the changes in the elastic modulus of the trabecular meshwork under different durations of elevated intraocular pressure. Results There was a significant difference in intraocular pressure between the experimental and control groups, indicating the successful establishment of the high intraocular pressure animal model. Specifically,the elastic modulus of the trabecular meshwork in the experimental groups at 2 weeks,3 weeks,and 4 weeks were (20. 55±5. 68) kPa,(23. 98±4. 42) kPa,and (28. 56±5. 94) kPa,respectively,significantly higher than their respective control groups. Additionally,there were statistical differences in the elastic modulus of the trabecular meshwork tissue among the three experimental groups at different time points. Conclusions The results suggest that with the sustained impact of high intraocular pressure,the elastic modulus of the trabecular meshwork tissue significantly increases,showing a positive correlation with the duration of exposure. This finding contributes to understanding the mechanical effects of elevated intraocular pressure on the trabecular meshwork, providing valuable insights into the physiological mechanisms influenced by high intraocular pressure and offering reference significance for studying the mechanisms of increased aqueous humor outflow resistance.
ratstrabecular meshworkhigh intraocular pressureatomic force microscopeelastic modulus
万方数据
维普
