In order to improve the extrusion module of the bio-printer can not achieve accurate temperature control, and the cantilever structure of the deposition platform is low precision. Based on TRIZ theory, function analysis and causal chain analysis of existing bio-printers are carried out to analyze the internal reasons of the problems. It is solved by 40 invention principles, contradiction matrix, clipping model, STC operator and smart man method. Four innovative solutions are proposed in this paper: the pipe contact surface is designed as water-cooled plate structure; The driving device of the nozzle is changed to 42 step motor and the driving mechanism is changed to ball screw; Using 19906 semiconductor refrigeration sheet instead of compressor, continuous refrigeration; The tower heat dissipation is used to heat the hot end of the cooling plate, which improves the cooling efficiency. After the four schemes are combined, the line width of the printed line is more stable.