Influence of EFI on cardiocyte lesion induced by H2O2
Objective To observe the protective effect of Epimedium Flavonoids Injection (EFI) on cardiocyte lesion induced by H2O2. Methods The cardiocytes of neonatal rats were cultured in vitro. The proliferation of cardiocytes was detected by MTT assayN the apoptosis of cardiocytes detected by flow cytometer and the ultrastructure of cardiocytes was observed by the transmission electron microscope ( TEM ). Results H2O2 increased the inhibitory rate of cardiocyte proliferation in a dose-dependent way. The cardiocyte apoptotic rate was (23. 94 ± 3. 52) % twelve hours after 100 μmol/L of H2O2 administration, which was higher than that of the control group [(1.98 ±1. 22) %]. The cardiocyte ultrastructure showed the apoptotic changes. EFI in doses of 100, 200 and 400 mg/L decreased the inhibitory rate of cardiocyte proliferation in a dose-dependent way, decreased significantly the cardiocyte apoptotic rate respectively [(15. 12 ±3.01) %, (9.38 ±3.14) % and (4. 49 ± 0. 52 ) %], and EFI also relieved the change of cardiocyte ultrastructure. Conclusion EFI has a protective effect on cardiocyte lesion induced by H2O2.
国家科技期刊平台
NSTL
万方数据
维普
