首页|HPLC测定小花黄堇中延胡索乙素的含量

HPLC测定小花黄堇中延胡索乙素的含量

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  • 国家科技期刊平台
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目的 建立用高效液相色谱法(HPLC)测定小花黄堇中主要有效成分延胡索乙素含量的方法.方法 色谱条件为色谱柱Gemini C18柱(250 mm×4.6 mm,5 μm);流动相为甲醇-0.1%磷酸溶液(75∶ 25);流速1.0 mL/min;检测波长280 nm;柱温25 ℃.结果 小花黄堇中延胡索乙素的平均含量为0.160%,延胡索乙素在0.193～2.128 μg范围内线性关系良好.平均回收率为98.55%,RSD为1.67%(n=6).结论 该方法操作简便,重复性较好,测定结果较为可靠,可用于测定小花黄堇中延胡索乙素的含量.
Quantitative determination of tetrahydropalmatine in Xiaohuahuangjin (Corydalis racemosa) by HPLC
Objective To establish a method for quantitative determination of tetrahydropalmatine in Xiaohuahuangjin (Corydalis racemosa) by HPLC. Methods The HPLC procedure was performed on the chromatographic column of Gemini C18 (250 mm × 4. 6 mm, 5 μm) at 25℃, the mobile phase was methanol-0. 1% phosphoric acid water solution (75:25). The flow velocity was 1.0 mL/min and the detection wavelength was 280 nm. Results The average content of tetrahydropalmatine in Xiaohuahuangjin was 0. 160% . Tetrahydropalmatine showed a good linear relationship at a range from 0. 193 μg to 2. 128 μg. The average recovery was 98. 55% (RSD = 1. 67%, n =6). Conclusion The method was simple and reliable with a good repeatability, which can be used for the quantitative determination of tetrahydropalmatine in Xiaohuahuangjin.

Xiaohuahuangjin ( Corydalis racemosa )tetrahydropalmatinequantitative determinationHPLC

蒋遐芝、叶锦霞、林珊、吴云丹、邹秀红、吴锦忠

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福建中医药大学中西医结合研究院,福建,350108

福建省永春县林业局

小花黄堇 延胡索乙素 含量测定 高效液相色谱法

陈可冀中西医结合发展基金资助项目福建省重点高校服务海西资助项目福建省中西医结合老年性疾病重点实验室开放课题资助项目

CKJ200900852008J1004-42

2010

北京中医药大学学报
北京中医药大学

北京中医药大学学报

CSTPCDCSCD北大核心
影响因子：1.568
ISSN：1006-2157
年,卷(期)：2010.33(10)
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