活血解毒方对干燥综合征小鼠颌下腺细胞凋亡的影响研究
Study on the effect of Huoxue Jiedu Formula on apoptosis of submaxillary gland cells in mice with Sj?gren syndrome
王东峰 1朱跃兰 2侯秀娟 2谷新怡 3胡海 4李方凯2
作者信息
- 1. 北京中医药大学第三附属医院 北京 100029
- 2. 北京中医药大学东方医院
- 3. 北京中医药大学东直门医院通州院区
- 4. 北京中医药大学附属护国寺中医医院
- 折叠
摘要
目的 探讨活血解毒方对干燥综合征小鼠颌下腺细胞凋亡相关因子B细胞淋巴瘤-2(Bcl-2)、存活蛋白(survivin)的影响.方法 以15 只健康C57BL/6J雌性小鼠为空白组.75 只非肥胖型糖尿病C57BL/6J雌性小鼠为干燥综合征模型小鼠,按随机数字表法分为模型组、白芍总苷胶囊组(0.233 g/kg)和活血解毒方低(15.925 g/kg)、中(31.850 g/kg)、高(62.335 g/kg)剂量组,每组 15只.空白组和模型组以蒸馏水灌胃,其他组按剂量灌胃相应药物,每日 1 次,连续 8 周.灌胃第 1、2、4、6、8 周后称量小鼠唾液分泌量,计算颌下腺指数,苏木素-伊红染色法观察小鼠颌下腺病理改变,采用蛋白质印迹法、实时荧光PCR法检测细胞凋亡相关因子Bcl-2、survivin表达.结果 6 组小鼠唾液分泌量组间、时间、交互比较差异有统计学意义(P<0.05).治疗 8 周后,与空白组比较,模型组唾液分泌量、颌下腺指数、Bcl-2、survivin蛋白及mRNA相对表达水平均降低,病理学评分升高(P<0.05).与模型组比较,白芍总苷胶囊组和活血解毒方低、中、高剂量组在治疗第 8 周后,唾液分泌量、颌下腺指数、Bcl-2、survivin mRNA相对表达水平均升高,病理学评分降低(P<0.05);活血解毒方中、高剂量组Bcl-2、survivin 蛋白相对表达水平均升高(P<0.05).与白芍总苷胶囊组比较,活血解毒方低剂量组第4、6 周唾液分泌量,颌下腺指数降低,病理学评分升高(P<0.05);活血解毒方高剂量组病理学评分降低(P<0.05);活血解毒方低、中剂量组Bcl-2 蛋白及mRNA相对表达水平降低(P<0.05).结论 活血解毒方可能通过调控颌下腺中Bcl-2、survivin细胞凋亡相关因子的表达,减轻颌下腺病变,增加干燥综合征模型小鼠颌下腺唾液分泌量.
Abstract
Objective To explore the effects of Huoxue Jiedu Formula on the expression of the apoptosis-related factors B-cell lymphoma-2(Bcl-2)and survivin in submaxillary gland cells in mice with Sjögren syndrome(SS).Methods Using 15 healthy C57BL/6J female mice as the blank control group.Seventy-five of non-obese diabetes mellitus female mice were classified as SS model.They were randomly assigned to the model group,total glucosides of paeony capsule group(TGP group,0.233 g/kg),Huoxue Jiedu Formula low-(15.925 g/kg),middle-(31.850 g/kg),and high-dose(62.335 g/kg)groups,with 15 mice in each group.The blank control and model groups received distilled water by gavage,and the other groups received respective drug doses by gavage once a day for 8 consecutive weeks.After 1,2,4,6,and 8 weeks of gavage,the salivary secretion volume of the mice was measured.The submaxillary gland index was calculated.Pathological changes in the submaxillary gland of mice were observed by hematoxylin-eosin staining.The expression of apoptosis-related factors Bcl-2 and survivin was detected by Western blotting and real-time fluorescence PCR.Results There was statistical significance in salivary secretion volume from aspects of between-group,time-point,and interaction effects(P<0.05).After 8 weeks of treatment,compared to the blank control group,salivary secretion volume,submaxillary gland index,and protein and mRNA expressions of Bcl-2 and survivin in the model group were decreased,while the pathological score grading increased(P<0.05).Compared to the model group,the TGP group and the Huoxue Jiedu Formula low-,medium-,and high-dose groups showed an increase in saliva secretion after 8 weeks of treatment and an increase in submaxillary gland index,Bcl-2,and survivin mRNA expression levels,and a decrease in pathological score levels.The protein expressions of Bcl-2 and survivin in the Huoxue Jiedu Formula medium-and high-dose groups were increased(P<0.05).Compared to the TGP group,salivary secretion volume and submaxillary gland index in the Huoxue Jiedu Formula low-dose group were lower at weeks 4 and 6,while the pathological score grading was higher(P<0.05).The pathological score grading in the Huoxue Jiedu Formula high-dose group was lower.The protein and mRNA expressions of Bcl-2 in the Huoxue Jiedu Formula low-and medium-dose groups were lower(P<0.05).Conclusion Huoxue Jiedu Formula may relieve the submaxillary gland lesions and increase the salivary secretion volume of the submaxillary gland in SS model mice by regulating the expressions of apoptosis-related factors such as Bcl-2 and survivin in the submaxillary gland.
关键词
活血解毒方/干燥综合征/颌下腺/细胞凋亡/小鼠Key words
Huoxue Jiedu Formula/Sjögren syndrome/submaxillary gland/cell apoptosis/mice引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据