Effect of radiotherapy on the expression of DNA methyltransferase in cervical cancer
Objective:To investigate the effect of radiotherapy on the expression of DNA methyltransferase(DNMT)1,3A,and 3B in cervical cancer(CIN)tissues,and to explore its role in radiotherapy resistance.Methods:The cervi-cal cancer tissues of 12 patients were selected before,during and after radiotherapy.The mRNA expression levels of DN-MT1,DNMT3A and DNMT3B in cervical cancer tissues after radiotherapy were detected by qPCR.Results:The relative expression levels of DNMT1 to β-ACTIN were 0.122 9±0.021 7,0.569 6±0.021 7 and 0.162 0±0.035 2 respec-tively before,during and after radiotherapy for cervical cancer.The expression of DNMT1 mRNA in cervical cancer tis-sues during and before radiotherapy was significantly different(P＜0.05),and the expression of DNMT1 mRNA in cer-vical cancer tissues during and after radiotherapy was significantly different(P＜0.05).The expression of DNMT1 mR-NA in cervical cancer tissues before and after radiotherapy was significantly different(P＜0.05).The relative expres-sion levels of DNMT3A to β-ACTIN mRNA were 0.001 2±0.000 1,0.002 1±0.000 1 and 0.001 4±0.000 1 re-spectively.There were significant differences in DNMT3A mRNA expression in cervical cancer tissues during radiothera-py and before radiotherapy(P＜0.05).The expression of DNMT3A mRNA in cervical cancer tissues during and after radiotherapy was significantly different(P＜0.05),however,the expression of DNMT3A mRNA before and after radio-therapy was not significantly different(P＞0.05).The relative expression of DNMT3B on β-ACTIN mRNA was 0.662 7±0.034 8,0.810 4±0.084 5 and 0.239 1±0.047 0 respectively.The mRNA expression of DNMT3B was significantly different in cervical cancer tissues during and before radiotherapy(P＜0.05).The expression of DNMT3B mRNA in cervical cancer tissues during and after radiotherapy was significantly different(P＜0.05),and the expression of DNMT3B mRNA in cervical cancer tissues before and after radiotherapy was significantly different(P＜0.05).Con-clusion:Radiotherapy changes the expression of DNMT1,DNMT3A and DNMT3B in cervical cancer tissues,which may play an important role in radioresistance.
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