放射治疗对宫颈癌DNA甲基转移酶表达的影响
Effect of radiotherapy on the expression of DNA methyltransferase in cervical cancer
徐晗 1高扬 2白静 3蔡志平 4邵国5
作者信息
- 1. 内蒙古科技大学包头医学院基础医学与法医学院,内蒙古 包头 014040;内蒙古科技大学包头医学院内蒙古低氧适应转化医学重点实验室
- 2. 包头医学院二附院呼吸科
- 3. 包头市肿瘤医院放射治疗科
- 4. 内蒙古科技大学包头医学院基础医学与法医学院,内蒙古 包头 014040
- 5. 内蒙古科技大学包头医学院内蒙古低氧适应转化医学重点实验室;深圳市龙岗区第三人民医院转化医学中心
- 折叠
摘要
目的:研究放射治疗对宫颈癌(cervical cancer,CIN)组织中DNA甲基转移酶(DNA methyltransferase,DNMT)1、3A和3B表达的影响,探讨其在放射治疗耐受中的作用.方法:选用12 例宫颈癌患者放射治疗前、中、后三个时期的组织,应用qPCR检测宫颈癌组织在放射治疗前期、中期和后期的DNMT1、DNMT3 A和DNMT3B mRNA的表达变化.结果:宫颈癌放射治疗前、中、后三个时期DNMT1 对β-actin mRNA的相对表达量分别是(0.122 9±0.021 7)、(0.569 6±0.021 7)和(0.162 0±0.035 2);放射治疗期间和放射治疗之前宫颈癌组织中DNMT1 mRNA的表达差异有统计学意义(P＜0.05),放射治疗期间和放射治疗之后宫颈癌组织中DNMT1 mR-NA的表达差异有统计学意义(P＜0.05),放射治疗之前和放射治疗之后宫颈癌组织中DNMT1 mRNA的表达差异有统计学意义(P＜0.05);DNMT3A对β-actin mRNA的相对表达量分别是(0.001 2±0.000 1)、(0.002 1±0.000 1)和(0.001 4±0.000 1),放射治疗期间和放射治疗之前宫颈癌组织中DNMT3A mRNA的表达差异有统计学意义(P＜0.05),放射治疗期间和放射治疗之后宫颈癌组织中DNMT3A mRNA的表达差异有统计学意义(P＜0.05),放射治疗之前和放射治疗之后宫颈癌组织中DNMT3A mRNA的表达差异无统计学意义(P＞0.05);DNMT3B对β-actin mRNA 的相对表达量分别是(0.662 7±0.034 8)、(0.810 4±0.084 5)和(0.239 1±0.047 0),放射治疗期间和放射治疗之前宫颈癌组织中DNMT3B mRNA的表达差异有统计学意义(P＜0.05),放射治疗期间和放射治疗之后宫颈癌组织中DNMT3B mRNA的表达差异有统计学意义(P＜0.05),放射治疗之前和放射治疗之后宫颈癌组织中DNMT3B mRNA的表达差异有统计学意义(P＜0.05).结论:放射治疗使宫颈癌组织中DNMT1,DNMT3 A和DN-MT3B表达发生变化,其可能在放射耐受中起到了作用.
Abstract
Objective:To investigate the effect of radiotherapy on the expression of DNA methyltransferase(DNMT)1,3A,and 3B in cervical cancer(CIN)tissues,and to explore its role in radiotherapy resistance.Methods:The cervi-cal cancer tissues of 12 patients were selected before,during and after radiotherapy.The mRNA expression levels of DN-MT1,DNMT3A and DNMT3B in cervical cancer tissues after radiotherapy were detected by qPCR.Results:The relative expression levels of DNMT1 to β-ACTIN were 0.122 9±0.021 7,0.569 6±0.021 7 and 0.162 0±0.035 2 respec-tively before,during and after radiotherapy for cervical cancer.The expression of DNMT1 mRNA in cervical cancer tis-sues during and before radiotherapy was significantly different(P＜0.05),and the expression of DNMT1 mRNA in cer-vical cancer tissues during and after radiotherapy was significantly different(P＜0.05).The expression of DNMT1 mR-NA in cervical cancer tissues before and after radiotherapy was significantly different(P＜0.05).The relative expres-sion levels of DNMT3A to β-ACTIN mRNA were 0.001 2±0.000 1,0.002 1±0.000 1 and 0.001 4±0.000 1 re-spectively.There were significant differences in DNMT3A mRNA expression in cervical cancer tissues during radiothera-py and before radiotherapy(P＜0.05).The expression of DNMT3A mRNA in cervical cancer tissues during and after radiotherapy was significantly different(P＜0.05),however,the expression of DNMT3A mRNA before and after radio-therapy was not significantly different(P＞0.05).The relative expression of DNMT3B on β-ACTIN mRNA was 0.662 7±0.034 8,0.810 4±0.084 5 and 0.239 1±0.047 0 respectively.The mRNA expression of DNMT3B was significantly different in cervical cancer tissues during and before radiotherapy(P＜0.05).The expression of DNMT3B mRNA in cervical cancer tissues during and after radiotherapy was significantly different(P＜0.05),and the expression of DNMT3B mRNA in cervical cancer tissues before and after radiotherapy was significantly different(P＜0.05).Con-clusion:Radiotherapy changes the expression of DNMT1,DNMT3A and DNMT3B in cervical cancer tissues,which may play an important role in radioresistance.
关键词
宫颈癌/放射治疗/DNA甲基转移酶/DNA甲基化Key words
cervical cancer/radiotherapy/DNA methyltransferase/DNA methylation引用本文复制引用
基金项目
国家自然科学基金(82060337)
深圳市龙岗区2021年度医疗卫生科技计划项目(LGKCYLWS2021000033)
出版年
2024
NETL
NSTL
万方数据