The mechanism of adipokine Chemerin regulating the migration and invasion of colon cancer cell lines
Objective:To investigate the effect of Chemerin on the invasion and migration of colon cancer cells and its mechanism.Methods:Colon cancer cell lines HCT116 and SW480 were treated with different concentrations of Chemerin(0,100,200,300,400,500 ng/mL),and the invasion and migration ability of cells were detected by Transwell method.HCT116 and SW480 cells were fur-ther studied and divided into 4 groups:pEGFP-N1 negative control group(vector control),si-Chemerin negative control group(si-NC),pEGFP-N1-Chemerin group and si-Chemerin group.After transfection,the invasion and migration ability of each group was detected.The mRNA levels of E-cadherin(E-cad),N-cadherin(N-cad)and vimentin were detected by qRT-PCR,and the protein levels of E-cad,N-cad and vimentin were detected by Western blot.Results:Exogenous Chemerin increased the invasion rate and migration number of colon cancer cells HCT116 and SW480(P＜0.05),which was dose-dependent,and cell migration number and invasion rate in pEGFP-N1-Chemerin group＞vector control group,si-NC group＞si-Chemerin group(P＜0.05).The expression levels of E-cad mRNA and protein in pEGFP-N1-Chemerin group＜vector control group,si-NC group＜si-Chemerin group(P＜0.05).The mRNA and protein expression levels of N-cad and vimentin in pEGFP-N1-Chemerin group＞vector control group,si-NC group＞si-Chemerin group(P＜0.05).Conclusion:Chemerin may promote colon cancer cell invasion and mi-gration by regulating epithelial-mesenchymal transition.
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维普
