Effects of electroacupuncture on the expression of HMGB1 and p-JNK proteins in the cingulate cortex of rats with cerebral ischemia
Objective:To observe the effect of electroacupuncture on the expression of high mobili-ty group protein 1(HMGB1)and phosphorylated c-Jun N-terminal kinase(p-JNK)in the anteri-or cingulate cortex of rats with cerebral ischemia,and to explore the protective effect and mechanism of electroacupuncture on the anterior cingulate cortex of rats with cerebral ischemia.Methods:Twenty-four male SD rats were randomly assigned into four groups,namely sham-operation,model,electroa-cupuncture,and sham-electroacupuncture groups,with 6 rats in each group.Cerebral ischemia was induced by blocking the right middle cerebral artery,creating a rat model of cerebral ischemia.Elec-troacupuncture was administered for 30 minutes,once daily for 14 days,targeting"Baihui"(GV20)and"Zusanli"(ST36)on the left side of the electroacupuncture group.In contrast,only the skin was penetrated in the sham-electroacupuncture group without activating the electroacupuncture device.Longa score was used to evaluate the neurological impairment of rats in each group.Nissl staining was used to observe the morphology and distribution of neurons in the right anterior cingulate cortex.The expression of HMGB1 and p-JNK protein in the right anterior cingulate cortex was detected by immu-nohistochemistry.Results:Compared with the sham-operation group,the neurological deficit scores of the model group and the sham-electroacupuncture group were increased(P＜0.01),the number of Nissl positive neurons in the right anterior cingulate cortex was decreased(P＜0.01),and the ex-pression of HMGB1 and p-JNK protein was increased(P＜0.01).Compared with the model group,the neurological deficit score of the rats in the electroacupuncture group decreased on the 7th and 14th day of cerebral ischemia(P＜0.05),the number of Nissl positive neurons increased(P＜0.01),and the expression of HMGB1 and p-JNK protein decreased(P＜0.01).Conclusion:Electroacu-puncture may reduce the damage of anterior cingulate cortex by inhibiting the overexpression of HMGB1 and p-JNK after cerebral ischemia.
Cerebral ischemiaAnterior cingulate cortexElectroacupunctureHigh-mobility group box 1Phosphorylated c-Jun N-terminal kinase
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维普
