Objective:To study the immunoregulatory effect of umbilical cord mesenchymal stem cells(UC-MSCs)on peripheral blood of patients with Sjogren's syndrome(SS).Methods:UC-MSCs were isolated and cultured from umbilical cord tissue of newborns,and the cell surface markers of UC-MSCs were detected by flow cytometry.Peripheral blood mononuclear cells(PBMCs)were collected from 20 SS patients in the active phase of the disease.Co-cultures of PBMCs from SS pa-tients with UC-MSCs were established,and cell viability was assessed using the CCK-8 assay.The optimal ratio of co-cultured cells was determined for subsequent experiments.Flow cytometry was used to analyze the changes in lymphocyte subsets in SS patient PBMCs after co-culture.Results:UC-MSCs reduced the proportions of peripheral blood CD3+T cells,CD3+CD8+T cells,and CD19+B cells in PBMC of SS patients,while the proportions of CD16+CD56+NK cells,CD3+CD4+T cells,and CD4+/CD8+ratio were not significantly affected.Conclusion:UC-MSCs can modulate the immune function of SS patients,thereby exerting a certain therapeutic effect on SS.
关键词
干燥综合征/脐带间充质干细胞/T淋巴细胞亚群/免疫功能
Key words
Sjögren's Syndrome/Umbilical cord mesenchymal stem cell/T lymphocyte subsets/Immune function
维普
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