摘要
目的:利用在线数据库和生物信息学的方法,研究BUB1B在胰腺腺癌(pancreatic ad-enocarcinoma,PAAD)组织中的表达水平及与预后和免疫细胞浸润的相关性.方法:利用GEPIA数据库分析BUB1B在各种癌症中的表达情况;GEPIA、Kaplan-Meier Plotter数据库检索BUB1B的表达与PAAD患者预后的相关性;随后利用String数据库构建BUB1B共表达基因的蛋白互作网络,并用David数据库分析其GO生物学功能及KEGG信号通路;最后使用TIMER数据库分析BUB1B基因的表达与免疫浸润细胞之间的相关性.结果:在PADD组织中,BUB1B表达水平高于正常组织(P<0.01);BUB1B的高转录水平与性别、年龄、种族、肥胖状况、饮酒习惯、慢性胰腺炎、淋巴转移和癌症阶段密切相关(P<0.05);BUB1B mRNA表达水平与PAAD预后和免疫细胞浸润相关(P<0.05).结论:BUB1B在PADD中高表达,与PAAD预后、免疫浸润相关,可作为胰腺癌诊断、预后、药物治疗的潜在标志物.
Abstract
Objective:To study the expression level,prognosis and correlation with immune cell infiltration of BUB1B in pancreatic adenocarcinoma (PAAD )by online database and bioinformatics. Methods:The expression of BUB1B in various cancers was analyzed by GEPIA database.The correla-tion between the expression of BUB1B and the prognosis of PAAD patients was searched by GEPIA and Kaplan-Meier Plotter database.Then,the protein interaction network of BUB1B co-expressed genes was constructed by using String database,and its GO biological function and KEGG signaling pathway were analyzed by using David database.Finally,the TIMER database was used to analyze the correla-tion between the expression of BUB1B gene and immune cell infiltrating cells.Results:The expression level of BUB1B in PADD tissues was higher than that in normal tissues (P＜0.01).The high tran-scription level of BUB1B was closely related to gender,age,race,obesity,drinking habits,chronic pancreatitis,lymph node metastasis and cancer stage (P＜0.05 ).The expression level of BUB1B mRNA was associated with prognosis and immune cell infiltration (P＜0.05).Conclusion:BUB1B is highly expressed in PADD,which is related to prognosis and immune infiltration,and can be used as a potential marker for diagnosis,prognosis and drug treatment of pancreatic cancer.
基金项目
中国营养学会-振东国人体质与健康研究基金(CNS-ZD2020-180)
国家自然科学基金(81703269)
国家自然科学基金(81973097)
黑龙江省自然科学基金(QC2018120)
维普
万方数据