The effect of miR-17-5p on biological behavior of lung adenocarcinoma HCC827 cells by regulating PKD2
Objective:To investigate the expression of miR-17-5p in lung adenocarcinoma cells,and to study the effects of miR-17-5p on proliferation,invasion,migration and apoptosis of lung adenocarcinoma cells and its molecular mechanism.Methods:The expression of miR-17-5p in human lung adenocarcinoma cell line HCC827 and human normal lung epithelial cell line BEAS-2B were detected by real-time fluorescent quantitative PCR.There were miR-17-5p mimics group,miR-17-5p inhibitor group and NC group in the experiment.The effects of miR-17-5p on proliferation,apoptosis,invasion and migration of lung adenocarcinoma cells were analyzed by CCK-8,flow cytometry and Transwell assay.Finally,PKD2 was predicted to be a potential downstream tar-get gene of miR-17-5p by Targetscan database.The interaction between miR-17-5p and PKD2 was verified by real-time fluorescence quantitative PCR and Western-blot experiments.Results:miR-17-5p was highly expressed in HCC827 cells (P＜0.001),and up-regulation of miR-17-5p significantly promoted the proliferation (P ＜0.05 ),invasion (P ＜0.01 ) and migration (P＜0.05)of lung adenocarcinoma cells,and inhibited apoptosis (P＜0.01 ).There was a direct binding site between miR-17-5p and 3'UTR of PKD2,and overexpression of miR-17-5p signifi-cantly promoted the expression of PKD2 at both mRNA and protein levels (P＜0.01 ).Conclusion:PKD2 was the direct target gene of miR-17-5p,and miR-17-5p affected the biological behavior of lung adenocarcinoma cells by regulating PKD2.
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维普
