Effect of cerium oxide nanoparticles on myocardial cell injury induced by Doxorubicin
Objective:To investigate the effect of cerium oxide nanoparticles(CeNPs)on doxorubicin(DOX)-induced myocardial cell injury and its possible mechanism in vitro.Methods:H9C2 cardiomyocytes were cultured with different concentrations of DOX for 24h to determine the optimal concentration to simulate DOX myocardial injury in vitro.On this basis,different concentrations of CeNPs with particle size of 20 nm and DOX were added for 24 h,and a normal control group was set up.CCK8 assay was used to measure the viability of cardiomyocytes,flow cytometry was used to measure the expression of reactive oxygen species(ROS),and Western-blot was used to measure the expres-sion of apoptosis-related proteins(Bcl-2,Bax).Results:DOX was treated with 1 μM for 24 h as the molding con-centration.Compared with normal control group,the survival rate of myocardial cells in model group decreased,ROS expression increased,anti-apoptotic protein Bcl-2 expression decreased,and pro-apoptotic protein Bax expression increased(P<0.05).Compared with the 1 μM DOX separate treatmentgroup,the survival rate of myocardial cellsin-creased,ROS expression decreased,the expression of anti-apoptotic protein Bcl-2 was up-regulated and the expres-sion of pro-apoptotic protein Bax was down-regulated after co-treatment with low concentration CeNPs(10 μg/mL、20 μg/mL、50 μg/mL)and 1μM DOX treatment(P<0.05);Co-treatment of CeNPs at a high concentration(100 μg/mL)and 1 μM DOX significantly decreased the cell survival rate(P<0.05)and increased the expression of ROS(P<0.05),while the expression of apoptosis-related proteins did not change significantly(P>0.05).Conclu-sion:Low concentration CeNPs(10,20,50 μg/mL)with particle size of 20 nm can alleviate oxidative stress and re-duce apoptosis to protect myocardial cell damage induced by DOX.High concentration of CeNPs(100 μg/mL)has no protective effect on DOX myocardial injury.
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