Exploring the mechanism of Dracocephalum moldavica L.protecting stroke based on network pharmacology and molecular docking
Objective:To investigate the protective effect of Dracocephalum moldavica L.on stroke based on network pharmacology.Methods:The effective components of Dracocephalum moldavica L.were searched by NCBI and CNKI.The effective targets of Dracocephalum moldavica L.were predicted by Pub-chem and swiss target prediction databases.The target genes related to cerebral stroke were searched by Gen-eCards and Omim databases.The Venn diagram was constructed to screen the intersection genes.Cytoscape was used to establish and visualize the network diagram of drugs,components,targets and diseases.String software was used to construct the protein network interaction diagram.GO enrichment and KEGG analysis were performed using the DAVID database.Results:A total of 23 effective components of Dracocephalum moldavica L.were found by searching the literature,and there were 1682 corresponding targets.The main molecules were NADPH oxidase 4(NOX4),xanthine dehydrogenase(XDH),myeloperoxidase(MPO),etc.Through PPI topology analysis,57 core targets were obtained;GO enrichment analysis showed that 443 biological processes(BP),104 molecular functions(MF)and 66 cellular components(CC)were involved.The results of KEGG showed that 143 signaling pathways were involved,including cancer pathway(hsa05200:pathways in cancer),PI3K-Akt signaling pathway(hsa04151:PI3K-Aktsignaling path-way),neuroactive ligand-receptor interaction(hsa05022:neuroactive ligand-receptor interaction)and so on.Conclusion:This study preliminarily revealed that Dracocephalum moldavica L.can treat stroke through a variety of targets and pathways,providing a theoretical basis for further scientific experiments.
万方数据
维普
