Objective:To explore whether the glycosides of cistanche can improve the learning and cognitive function of sleep deprivation model rats by regulating synaptic plasticity.Methods:Fifty male Wistar rats were randomly divided into blank control group(Control),platform control group(PC),model group(Model),glycosides of cistanche group(GCs)and estazolam group(Est).The sleep deprivation model of rats was prepared by modified multi-platform water environment method.Morris water maze and open field test were used to detect the spatial cognitive function and emo-tional changes of rats.Nissl staining was used to observe the changes of morphology and quantity of nerve cells in hipp-ocampal CA1 area of rats.Western blot and RT-PCR were used to detect the expression levels of synaptophysin(SYN),postsynaptic membrane dense substance 95(PSD-95)and brain-derived neurotrophic factor(BDNF).Results:Morris water maze results showed that compared with the Control group,the learning and cognitive ability of rats in the Model group was significantly decreased(P＜0.05),and the learning and memory function of rats was improved after GCs treatment(P＜0.05).The results of the open field test showed that compared with the Control group,the movement distance and standing times of the Model group were significantly increased(P＜0.05),the resting time was significantly shortened(P＜0.05),and the anxiety was increased,while after GCs treatment,the movement distance and standing times decreased(P＜0.05),the resting time increased(P＜0.05),and the anxiety was improved.The results of Western blot and RT-PCR showed that the protein and gene expression levels of BDNF,SYN and PSD-95 in the Model group were significantly decreased(P＜0.05),while after treatment with GCs,the expression levels of BDNF,SYN and PSD-95 were significantly up-regulated(P＜0.05).Conclusion:Glycosides of cistanche may af-fect synaptic plasticity by regulating the expression of synaptic related markers,thereby improving the learning and cog-nitive function of sleep deprivation model rats.
关键词
肉苁蓉总苷/海马/睡眠剥夺/学习记忆/焦虑/突触可塑性
Key words
Glycosides of cistanche/Hippocampus/Sleep deprivation/Learning and memory/Anxiety/Synaptic plasticity
维普
万方数据