Effect of ginsenoside Rb1 on alleviating lipopolysaccharide induced acute lung injury
Objective:To explore the protective effect and mechanism of ginsenoside Rb1(GsRb1)on lipopolysaccharide(LPS)-induced acute lung injury(ALI).Methods:C57 BL/6 mice were randomly divided into control group,model group,GsRb1 administration group(low,medium and high dose groups).LPS was given intratracheally to establish the model.The administration group was given intraperitoneal injection of different doses of GsRb1 pretreatment 3 days before modeling.Af-ter 12 hours of modeling,the alveolar lavage fluid was collected and the lung tissue was taken out.The lung wet/dry ratio was calculated.The levels of interleukin-6(IL-6),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in lavage fluid and the activity of myeloperoxidase(MPO)in lung were detected by kit.The expression of nuclear factor-κB(NF-κB)protein in lung tissue was detected by Western blot.Results:High dose GsRb1 could significantly reduce the wet/dry ratio of lung tissue(P＜0.05).Medium and high doses of GsRb1 could significantly reduce the MPO activity in lung tissue(P＜0.05).High dose GsRb1 could significantly reduces the levels of IL-1βand TNF-αin alveolar lavage fluid of mice(P＜0.05).High dose GsRb1 could significantly reduce the ex-pression of NF-κB p65 protein in lung tissue(P＜0.05).Conclusion:GsRb1 may reduce inflam-mation response and inhibit LPS-induced ALI in mice by regulating the NF-κB pathway.
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维普
