Analysis of correlation between ALEX1 level and clinical features in breast cancer tissues
Objective:To explore the correlation between ALEX1 level and clinical features of breast cancer his-topathologic detection by immunohistochemistry.Methods:ALEX1 positive rate,protein and mRNA levels were detected by immunohistochemistry,western blotting and PCR in 77 cases of breast cancer and 2 cm adjacent specimens,and the correlation between ALEX1 and clinicopathologic features was analyzed.Results:ALEX1 showed yellow and yellowish brown expression in the cell membrane of 77 breast cancer tissues and adjacent tissues.ALEX1 positive expression in45 breast cancer tissues was 58.44%,and positive rate in 61 adjacent tissues was 79.22%.There was significant differ-ence between groups(χ2=4.199,P＜0.05).The protein expression of ALEX1 in 77 breast cancer tissues and adja-cent tissues was 2.05±0.55 and 4.11±0.69,respectively.The protein content of ALEX1 in breast cancer tissues was lower than that in adjacent tissues,and there was significant difference between groups(t=20.85,P＜0.001).The expression of ALEX1mRNA was 1.00±0.00 and 0.45±0.08 in 77 paracancer tissues and breast tissues,and there were significant differences in the expression of ALEX1mRNA in different tissues(t=60.330,P＜0.001).ALEX1 ex-pression was correlated with pathological type,pathological grade and lymph node metastasis(P＜0.05),but not with other indicators(P＞0.05).The relationship between ALEX1 level and clinicopathology was observed according to the 3 classification boundaries.The decreased level of ALEX1 was correlated with invasive ductal carcinoma and pathologi-cal gradeⅡtoⅢof breast cancer patients(P＜0.05).The AUC area of ALEX1 in breast cancer was 0.646,and the specificity and sensitivity were 55.42%and 65.02%,respectively,indicating the significance of ALEX1 expression de-tection in the pathological diagnosis of breast cancer.Conclusion:The expression level of ALEX1 in paracancer tissues is significantly higher than that in breast cancer tissues,and the decreased expression of ALEX1 is associated with in-creased lymph node metastasis and pathological grade of breast cancer.
万方数据
维普
