Bioinformatics analysis of TENM1 and its effect on the proliferation and migration of MDA-MB-231 breast cancer cells
Objective:To study the bioinformatics-driven expression and prognostic significance of TENM1 in breast cancer(BRCA)and its effect on the proliferation and migration of MDA-MB-231 cells.Methods:TENM1 expression profiles of breast cancer tissue samples from the TCGA database were download for differential expression analysis.Survival analysis,univariate and multivariate Cox regression analysis were performed,and a Nomogram prediction model was established to validate the independent prognostic value of TENM1.Gene set en-richment analysis was used to evaluate dysregulated signaling pathways associated with TENM1 expression.After in-terfering and overexpressing TENM1 in MAD-MB-231 cells,proliferative capacity of cells was detected using CCK8 and clonogenic assays,and migratory capacity was detected using the Transwell migration assay.Results:TENM1 expression was decreased in breast cancer tissues comparing to normal samples.Results of survival analy-sis,univariate and multivariate Cox regression analysis and Nomogram prediction modeling indicated that TENM1 might be an independent prognostic factor for BRCA.GSEA analysis results showed that TENM1-related signaling pathways were selectively enriched at cell division and cell cycle.Suppression of TENM1 increased the proliferation and migration of MDA-MB-231 cells,as shown by CCK8,clonogenic assay and Transwell migration assay.Con-versely,overexpression of TENM1 inhibited proliferation and migration.Conclusion:Down-regulated TENM1 ex-pression is in breast cancer tissues could inhibit the proliferation and migration of MDA-MB-231 cells.TENM1 might be a valuable prognostic molecular marker for BRCA and a promising target for future treatment of BRCA.
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