The mechanism of NapA protein inducing macrophages to secrete chemokine monocyte chemoattractant protein 1 and interleukin 8
Objective:To investigate the mechanism of H.pylori NapA protein inducing the secre-tion of monocyte chemoattractant protein-1 (MCP-1 )and interleukin-8 (IL-8)in macrophages.Methods:Macrophages were stimulated by NapA,then chemokines MCP-1 and IL-8 in supernatant were detected through ELISA.Macrophages were pre-incubated with C29,ST2825,SB203580,SP600125 and PDTC for 1 h to inhibit the activity of TLR2,MyD88,p38,c-Jun and NF-κB,re-spectively.Then NapA protein was added and incubated for 4 h.The supernatant was collected and the expression levels of MCP-1 and IL-8 were examined.Results:The expression levels of MCP-1 and IL-8 in macrophages stimulated by H.pylori NapA protein were significantly higher than those in untreated group.When the activity of TLR2 was inhibited by C29,the expression of MCP-1 and IL-8 induced by NapA protein decreased.Inhibition of MyD88 NF-κB and p38 decreased the abili-ty of macrophages to secrete MCP-1 and IL-8 induced by NapA protein.However,inhibition of c-Jun did not affect the secretion of MCP-1 and IL-8 by macrophages induced by NapA protein.Con-clusion:H.pylori NapA protein induces macrophages to secrete chemokines MCP-1 and IL-8 via TLR2/MyD88/NF-κB pathway.
H.pyloriNapA proteinToll-like receptor 2ChemokinesMonocyte chemoattractant protein 1Interleukin 8
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