Effects of miR-152-3p on the proliferation,migration,invasion and apoptosis of colon cancer by regulating DNMT1
Objective:To investigate the regulatory mechanism of miR-152-3p and DNA Methyltransferases(DN-MT1)in colon cancer cells.Methods:qRT-PCR was used to detect the expression of miR-152-3p and DNMT1. Cell proliferation assay,colony formation assay,scratch healing assay and Transwell assay were used to detect the prolif-eration,migration and invasion ability of cancer cells in each treatment group.Dual luciferase assay was used to detect the targeting relationship between miR-152-3p and DNMT1.Western blot assay was used to detect the protein expres-sion of DNMT1.Flow cytometry assay was used to detect apoptosis rate of cells.Results:MiR-152-3p was signifi-cantly downregulated while DNMT1 was highly expressed in colon cancer cells.Overexpression of miR-152-3p inhibi-ted the proliferation,migration and invasion of colon cancer cells.MiR-152-3p could inhibit the expression of DN-MT1,thereby inhibiting the proliferation,migration and invasion of colon cancer cells.Overexpression of miR-152-3p could target DNMT1 and promote cell apoptosis.Conclusion:MiR-152-3p could downregulate the expression of DNMT1,thereby inhibiting the development of colon cancer cells.
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