Effect of general cistanosides on synaptic plasticity induced by Aβ1-42 oligomers in HT22 cells
Objective:To investigate the effect of general cistanosides(GCs)on synaptic plasticity of Alzheimer s disease(AD)cell model constructed by Aβ1-42 oligomer-induced mouse hippocampal neuron cell line(HT22)cells.Methods:HT22 cells were divided into control group,model group,blank rat serum group and GCs administra-tion group.The morphology,viability and expression levels of synapse-related mRNA and proteins in each group were recorded.Results:Compared with the control group,the synaptic length of the model group was significantly shortened,the cell body was small,the morphology was poor,the apoptotic cells were increased,the cell viability was decreased,the mRNA levels of brain-derived neurotrophic factor(BDNF),postsynaptic density protein(PSD-95)and N-methyl-D-aspartate receptor 1(NMDAR1)were significantly decreased(P＜0.05),and the protein expression lev-els of PSD-95 and NMDAR1 were significantly decreased(P＜0.05).Compared with the model group and the blank rat serum group,the synaptic length,density and cell viability(P＜0.05)of the GCs administration group were re-stored,the number of apoptotic cells was decreased,the mRNA expression levels of BDNF,synaptophysin(SYN),PSD-95 and NMDAR1 were significantly increased(P＜0.05),and the expression levels of NMDAR1 and PSD-95 pro-teins were significantly increased(P＜0.05).Conclusion:Medicated serum of GCs can significantly improve the syn-aptic plasticity and pathological process of AD cell models.
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