Effects of fluoride exposure on myocardial apoptosis in rats
Objective:To explore the toxic effect of chronic fluorosis on myocardium and its effect on JNK-mediated apoptosis.Methods:Wistar rats were randomly divided into 4 groups:control group and different concentrations of sodium fluoride solution groups(50,100,200 mg·L-1 NaF).The rats were free to drink,and the control group drank deionized water.After 4 months,the rat mod-el of chronic fluorosis was obtained.The effects of different concentrations of NaF on the myocardial tissue structure of rats were compared by HE staining,and the damage of myocardial tissue was ob-served.The expression of apoptosis-related proteins in myocardial tissue was detected by Western blot,and the effect of NaF on the expression of apoptotic proteins was observed,including p-JNK,Bcl-2,Bax and Caspase-3 protein expression.Results:After 4 months,a rat model of chronic flu-orosis was established.The myocardial tissue of rats in each group showed muscle fiber structure disor-der,muscle fiber breakage,muscle cell atrophy,muscle gap widening,and disappearance of trans-verse striations.The results of Western blot showed that compared with the control group,the expres-sion of Bcl-2 protein in myocardium of rats with chronic fluorosis was significantly decreased(P＜0.05),and the expression of Bax,Caspase-3 and p-JNK protein was significantly up-regulated(P＜0.05).Conclusion:Chronic fluorosis may cause myocardial apoptosis by activating JNK signa-ling pathway,and apoptosis is involved in the damage of fluoride to myocardial tissue.
万方数据
维普
