Transcriptome analysis of MCP in the treatment of rheumatoid arthritis with pulmonary fibrosis
Objective:To investigate the therapeutic effect and potential mechanism of modified citrus pectin(MCP)in the treatment of rheumatoid arthritis with interstitial lung disease(RA-ILD).Methods:The RA-ILD mouse model was established and divided into control group,model group and MCP treatment group.Lung tissue was taken for pathological section.The other part of lung tissue was subjected to transcriptome sequencing and subsequent bioinformatics analysis.Results:MCP could improve the pathological changes of pulmonary fibrosis in RA-ILD mice.A total of 23 core genes for drug intervention were screened,of which the expression of 9 homologous genes in humans was consistent with the expression trend in mice,which were LCK,THY 1,GZMA,CXCR3,LR10RA,CCL5,CTLA4,CD19,and CD5.The enrichment functions and pathways revealed that pathways such as T cell activation regulation,cell adhesion regulation,immune receptor activity,Th1 7 cell differentiation,and Th1 and Th2 cell differentiation played an important role in MCP treatment of fibrosis.Conclusion:(1)MCP has a definite therapeutic effect on RA-ILD mice,which can significantly improve the severity of pulmonary fibrosis and the degree of inflammatory cell infiltration in lung tissue;(2)There are 23 core genes that play a key role in the treatment of diseases,including 9 human homolo-gous genes;(3)Core genes play a role through T cell activation regulation,cell adhesion regulation,immune receptor activity,Th17 cell differentiation,Th1 and Th2 cell differentiation.
万方数据
维普
