Protective effect of APX-115 free base on H9c2 cells against high concentration of glucose
Objective:To investigate the protective effect of Nox inhibitor APX-115 free base on H9c2 cells against high concentration of glucose.Methods:The proliferative activity of H9c2 cells af-ter pretreatment with different concentrations of APX-115 free base was detected by CCK8 assay.The expression of Nox4 protein was detected by Western blot and immunofluorescence.The morphological changes of H9c2 cells were observed.Results:Compared with the Model group,whrn the concentra-tion of APX-115 free base was 8 μmol/L,the cell proliferation activity decreased,and the cell prolif-eration activity increased at other concentrations.The expression of Nox4 protein in each treatment group of APX-115 free base was down-regulated.The cell morphology of the APX-115 free base 5μmol/L treatment group returned to spindle shape,the cell distribution was gradually uniform,the in-tercellular space was reduced,and the number of cells basically returned to normal level.Conclusion:When APX-115 free base is 5 μmol/L,it has the best protective effect on H9c2 cells induced by high glucose.
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