包头医学院学报2024，Vol.40Issue(12) ：13-17.DOI:10.16833/j.cnki.jbmc.2024.12.003

CCN1对食管癌细胞APRIL/BAFF表达的影响

Effect of CCN1 on APRIL/BAFF expression in esophageal cancer cells

邢玲玲 刘琳 江振宇 柴建原 武金宝
包头医学院学报2024，Vol.40Issue(12) ：13-17.DOI:10.16833/j.cnki.jbmc.2024.12.003
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CCN1对食管癌细胞APRIL/BAFF表达的影响

Effect of CCN1 on APRIL/BAFF expression in esophageal cancer cells

邢玲玲 1刘琳 2江振宇 2柴建原 2武金宝2
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作者信息

  • 1. 内蒙古科技大学包头医学院,内蒙古 包头 014040;内蒙古科技大学包头医学院第二附属医院内蒙古消化病研究所
  • 2. 内蒙古科技大学包头医学院第二附属医院内蒙古消化病研究所
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摘要

目的:探究CCN1蛋白在食管癌中对增殖诱导配体(APRIL)和B细胞刺激因子(BAFF)所起到的作用.方法:用质粒转染技术在细胞株KYSE15 0及O E-19中过表达CCN1,Western blot验证其转染效果,同时检测APRIL和BAFF的表达情况.结果:在KYSE150细胞中过表达CCN1后,APRIL/BAFF高表达(P＜0.05);在OE19细胞中过表达CCN1后,APRIL/BAFF低表达(P＜0.05).结论:CCN1在食管鳞癌细胞KYSE150中对APRIL/BAFF为激活作用,在食管腺癌细胞OE19中对APRIL/BAFF为抑制作用.

Abstract

Objective:To investigate the role of CCN1 protein in a proliferation-inducing ligand(APRIL)and B-cell activating factor(BAFF)in esophageal cancer.Methods:CCN1 was overex-pressed in KYSE150 and OE-19 cell lines by plasmid transfection technique.The transfection effect was verified by Western blot,and the expression of APRIL and BAFF was detected.Results:APRIL/BAFF was highly expressed after overexpression of CCN1 in KYSE150 cells(P＜0.05),and APRIL/BAFF was lowly expressed after overexpression of CCN1 in OE19 cells(P＜0.05).Conclu-sion:CCN1 activates APRIL/BAFF in esophageal squamous cell carcinoma cell line KYSE150 and in-hibits APRIL/BAFF in esophageal adenocarcinoma cell line OE19.

关键词

CCN1/增殖诱导配体/细胞刺激因子/食管癌

Key words

CCN1/A proliferation-inducing ligand/B-cell activating factor/Esophageal cancer

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出版年

2024
包头医学院学报
内蒙古科技大学包头医学院

包头医学院学报

影响因子：0.543
ISSN：1006-740X
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