Protective effect of Toll-like receptor-4 inhibitor TAK-242 on hippocampal inflammatory injury in rats with post-stroke depression
Objective:To investigate the protective mechanism of TAK-242 on hippocampal inflammatory injury in rats with post-stroke depression.Methods:A total of 54 male 3-month-old SD rats were divided into sham opera-tion group(n=18),post-stroke depression(PSD)model group(n=18)and TAK group(n=18).The middle cer-ebral artery occlusion(MCAO)model was established by suture method,and then chronic unpredictable mild stress(CUMS)stimulation was performed for 3 weeks to establish the PSD model.After the PSD model was successfully es-tablished,the TAK group was intraperitoneally injected with diluted TAK-242 0.3 mg/kg,which was performed on the 5th,10th,15th and 20th day of CUMS,respectively.The level of IL-1β in peripheral blood was detected by enzyme-linked immunosorbent assay.Results:Compared with the sham operation group,the body weight,sucrose preference index and movement(LA)of the PSD group and the TAK group were lower than those of the sham operation group,and the level of IL-1 β was significantly increased(P＜0.05).Compared with PSD group,the body weight,sucrose pref-erence index and LA of rats in TAK group were increased,and the expression level of IL-1β was decreased(P＜0.05).Conclusion:TAK-242 can reduce the expression of IL-1 β in peripheral blood by inhibiting TLR4 sig-naling pathway,thus exerting a neuroprotective effect on hippocampal inflammatory injury in rats with post-stroke de-pression,and providing new ideas for clinical prevention and treatment of post-stroke depression.
万方数据
维普
