Objective To explore the sensitizing effect and molecular mechanism of myricetin via β-catenin on erlotinib a-gainst non-small cell lung cancer.Methods A549 cells were divided into the control group,the erlotinib group and the myricetin group.The expression of β-catenin with time and dose was detected by Western blot.The siβ-catenin was constructed and trans-fected A549 to knockdown β-catenin expression.The colony formation assay was applied to observe the colony formation rate after erlotinib treatment.Erlotinib and myricetin alone and combined treatment groups were constructed,apoptosis status was detected by Hoechst 33258,and apoptosis-related protein expression was detected by Western blot.Results β-catenin expression in A549 was significantly induced by erlotinib with time-dependent and dose-dependent effects,and the sensitivity of A549 to erlo-tinib was enhanced by inhibition of β-catenin.The expression of β-catenin was significantly inhibited by myricetin.The apoptosis rate and the expression of apoptosis-related proteins were significantly increased in the combination of myricetin and erlotinib com-pared with erlotinib alone.Conclusion Myricetin enhanced the sensitivity of erlotinib against non-small cell lung cancer by pro-moting apoptosis through β-catenin,which provides experimental basis and new therapeutic ideas for the combined use of myrice-tin and erlotinib to resist drug resistance.
关键词
人非小细胞肺癌/厄洛替尼/β-catenin/杨梅素
Key words
human non-small cell lung cancer/erlotinib/β-catenin/myricetin
维普
万方数据