Objective To investigate the efficacy of pH-sensitive carrier(PEPB36/35)loaded with triclosan(TCS)in the form of drug-loaded nanoparticles(PEPB36/35@TCS)for the eradication of Methicillin-resistant Staphylococcus aureus(MRSA)biofilms.Methods pH-sensitive polymers(PEPBx/y)were prepared by using nanoprecipitation techniques.The dissociation constant(pKa)of PEPBx/y were determined through acid-base titration.The effectiveness of PEPBx/y in eradicating MRSA bio-films were examined by using Confocal Laser Scanning Microscopy,from which the optimal carrier PEPB36/35 was selected.PEPB36/35@TCS was prepared by using nanoprecipitation and its pharmacological properties were examined.The in vitro and in vivo efficacy of PEPB36/35@TCS in eradicating MRSA biofilms and internal bacteria were assessed by using agar plate colony counting,crystal violet staining,and mouse dorsal wound MRSA infection model.Results The pKa value of PEPB36/35 was found to be 5.6.The MRSA biofilm eradication rate by PEPB36/35 was approximately 90％.The particle size of PEPB36/35@TCS was(190.6±4.05)nm,with a zeta potential of(0.317±0.18)mV and a Polydispersity Index(PDI)of(0.172±0.017).In a-cidic environments,the survival rate of MRSA biofilm internal bacteria,residual biofilm mass,and wound bacteria survival rate significantly reduced(P＜0.01)in the PEPB36/35@TCS group.Conclusion PEPB36/35@TCS demonstrated effective eradication of MRSA biofilms and internal bacteria in acidic environments,and can play a role in promoting the healing of infected wounds.
维普
万方数据