Study on the quality standard of Perillae caulis and Gleditsiae sinensis fructus
Objective To establish the quality standards of Perillae caulis and Gleditsiae sinensis fructus.Methods The medicinal properties and powder microscopic characteristics of 10 batches of Perillae caulis and Gleditsiae sinensis fructus from different origins were conducted respectively.Qualitative identification was carried out using thin layer chromatography(TLC).Their moisture and total ash were measured separately.Rosmarinic acid content in Perillae caulis was determined using HPLC,and the acid-insoluble ash and leachate of Gleditsiae sinensis fructus were determined.Results The properties of Perillae caulis and Gleditsiae sinensis fructus are consistent with the description in the Chinese Pharmacopoeia,and their microstructure charac-teristics were clearly defined.In the TLC identification,the test sample and the control sample showed the same color spots at the same location.The moisture and total ash of Perillae caulis were≤12.0%and 8.0%,respectively.The content of rosmarinic acid in Perillae caulis was≥0.10%.The moisture,total ash content,and acid insoluble ash of Gleditsiae sinensis fructus were≤13.0%,8.0%and1.0%,respectively.The content of water cooled leaching extracts,water hot leaching extracts,alcohol cooled leaching extracts,and alcohol hot leaching extracts were≥30.0%.Conclusion The present study developed quality standards for Perillae caulis and Gleditsiae sinensis fructus,which provided a reference for the revision of quality standards for these two herbs.
万方数据
维普
