Isolation,identification and antibacterial activity of entomogenous fungi from Cryptotympanaatrata
Objective To isolate and identify entomogenous fungi from Cryptotympanaatrata,and explore preliminarily their species diversity and antibacterial activity.Methods Tissue sectioning method was used to isolate and purify the entomogenous fungi of the Cryptotympanaatrata,morphological characteristics and ITS sequence identification were used to obtain the fungal species and genera,and MEGA7 software was used to construct a phylogenetic tree using adjacency method.Chloramphenicol as a positive control drug,filter paper method was used to screen fungi with antibacterial activity.Results Fifteen fungi were isola-ted from the Cryptotympanaatrata,and eleven fungi belonged to seven genera,including Aspergillus sp.,Alternaria sp.,Clados-porium sp.,Fusarium sp.,Penicillium sp.,Stagonospora sp.and Yarrowia sp..ZL8,ZL11 and ZL14 had significant antibac-terial activity against Staphylococcus aureus,Escherichia coli,Proteus and Candida albicans,and were identified as Cladospori-um haloolerans,Penicillium sp.and Stagonospora sp.Conclusion The study preliminarily explored the types of entomophytic fungi in the grasshopper,laying a foundation for the research on their entomophytic fungal resources and secondary metabolites.
万方数据
维普
