Study on constructing a bimetallic-based magnetic resonance imaging nanoprobes and its contrast ability
Objective To synthesize a novel gadolinium-vanadium-based nanoparticles (GdVN)as T1 magnetic resonance imaging nanoprobes by using the vanadium ion-doped method,and explore its application as a T1 magnetic resonance imaging nanoprobe;to optimize the contrast ability of GdVN via adjusting the feed ratio of precursors,and evaluate the bio-safety of Gd-VN.Methods Firstly,the morphology and size of GdVN were measured via the transmission electron microscopy (TEM)and the dynamic light scattering (DLS)detector;Subsequently,the structure and composition of GdVN were analyzed with the X-ray diffraction (XRD),Fourier transform infrared spectroscopy (FT-IR),and X-ray photoelectron spectrometry (XPS),respective-ly. Secondly,the T1 relaxivity of GdVN was measured with a 0.5 T magnetic resonance imaging scanner to screen out the GdVN with high T1 relaxivity. Finally,the MTT experiments were performed for evaluating the biological safety of nanoprobes. Results GdVN nanoprobes had been successfully prepared by using vanadium ion-doped technology. In addition,the contrast ability of GdVNs had been optimized via adjusting the feed ratio of precursors. When the feeding ratio of gadolinium acetylacetonate to va-nadium acetylacetonate was up to 1:4,the obtained GdVN-2 exhibited regular fusiformis shaped and excellent dispersibility,and the size was approximately 90 nm. Meanwhile,GdVN-2 nanoprobes exhibited the high T1 relaxivity (r1=17. 76 mM-1 s-1 ),and the cytotoxicity assay demonstrated that GdVN-2 nanoprobe possessed the excellent bio-safety,which was beneficial to the ex-cellent potential of GdVN-2 as T1 magnetic resonance imaging nanoprobes in vivo. Conclusion In this study,GdVN-2 nano-probes with uniform morphology exhibits the excellent contrast ability and biosafety,providing the technical support for achieving early and accurate diagnosis of tumors.
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维普
