Preparation of Fe3 O4 composite nanosystem mediated by MRI visualization and its application in tumor photothermal therapy
Objective To investigate the preparation of novel Fe3O4/780 composite nanoparticles (Fe/780 NPs)by emulsifi-cation-solvent evaporation method for guiding magnetic resonance imaging (MRI)visualization-guided combined photothermal therapy of tumors. Methods The particle size distribution and morphology of Fe/780 NPs were determined by dynamic light scattering (DLS)and transmission electron microscopy (TEM). Near-infrared thermal imaging was used to evaluate the photo-thermal conversion effect of Fe/780 NPs. Hemolysis test was used to evaluate the biocompatibility of Fe/780 NPs. The antitumor activity in vitro was evaluated by detecting intracellular reactive oxygen species (ROS),cell alive/dead double staining and MTT assay. A 7.0 T small animal magnetic resonance imaging instrument was used to analyze the MRI imaging effect of the nanoparti-cles in the tumor sites. The anti-tumor effect of the preparations in vivo was evaluated by establishing a 4T1 tumor cell ectopic transplantation tumor model.Results The highly efficient co-loaded IR780 and MNPs delivery nanosystem was successfully pre-pared by emulsification-solvent volatilization method,and the nanoparticle size distribution was uniformed. The addition of MNPs made the photothermal conversion performance of the system significantly better than that of the single ones,reducing the dose,enhancing the biological compatibility,and making it a mild heating process to avoid the generation of excessive heat. It showed good anti-tumor activity in vivo and in vitro anti-tumor experiments,which could be targeted to tumor sites and has good MRI im-aging ability.Conclusion Fe/780 NPs can be used for MRI visualization mediated photothermal therapy of tumors,which can make the diagnosis and treatment of cancer more accurate.
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