Experimental study of salidroside affecting podocyte damage induced by high glucose by regulating miR-92b-5p
Objective To investigate the effect of salidroside on podocyte damage induced by high glucose (HG)and its pos-sible mechanism.Methods The mouse podocytes MPC5 were cultured in vitro. After MPC5 cells induced by HG were inter-vened with different doses of salidroside,the cell proliferation activity was detected by CCK-8 method;apoptosis was detected by flow cytometry,and the expression of cleaved-caspase-3 and Bax protein in the cells were detected by Western blotting. Colori-metric method was used to detect the amount of LDH leakage,the content of MDA and the activity of SOD in cells,and qRT-PCR method was used to detect the expression of miR-92b-5p in cells. After MPC5 cells that were transfected with miR-92b-5p mimics or inhibitors were intervened with salidroside and/or HG,the same method described above were used to detect cell proliferation activity,apoptosis rate,the expression of cleaved-caspase-3 and Bax protein,the amount of LDH leakage,the content of MDA and the activity of SOD in cells.Results Slidroside increased the proliferation activity of MPC5 cells and the activity of SOD in cells induced by HG(P<0. 05 or<0. 01),but reduced the rate of apoptosis,the expression of cleaved-caspase-3 and Bax pro-tein,the amount of LDH leakageand the content of MDA in cells(P<0. 05 or<0. 01). At the same time,salidroside promoted the expression of miR-92b-5p in MPC5 cells induced by HG(P<0. 05 or<0. 01). Up-regulation of miR-92b-5p increased the proliferation activity of MPC5 cells and the activity of SOD in cells induced by HG,but reduced the rate of apoptosis,the expres-sion of cleaved-caspase-3 and Bax protein,the amount of LDH leakageand the content of MDA in cells(P<0. 01). Down-regulation of miR-92b-5p reversed the effects of salidroside on the proliferation,apoptosis and oxidative stress of MPC5 cells induced by HG (P<0.01).Conclusion Salidroside may increase the proliferation of MPC5 cells induced by HG and inhibit cell apoptosis and oxidative stress by up-regulating miR-92b-5p.
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