Screening of bactericidal and bacteriostatic monomer molecules in Astragali Raidx and detection of differentially expressed genes
Objective To screen effective monomeric molecules against Staphylococcus aureus(SA)in Astragali Raidx,and to screen differentially expressed genes following the action of effective monomeric molecules.Methods In vitro inhibition assay(MIC)of SA was conducted for 6 monomer molecules from Astragali Raidx,and the monomer molecules with good inhibition effect on SA were screened.In vitro bactericidal assay(MBC)was conducted to verify the sterilization function of the monomer molecules with good inhibitory effect on SA,and the optimal bactericidal concentration was determined by setting different con-centration groups.The samples of SA after the action of the effective bactericidal inhibitory monomer molecules were collected with the unspiked SA,and the transcriptome analysis was performed by RNA-seq technology to compare the differentially ex-pressed genes after the action of the effective monomer molecules.Results The MIC experiment screened 3 monomer molecules with inhibitory effect on SA,among which Xanthohumol had the best inhibition effect,Formononetin had good inhibition effect,Naringenin had inhibition effect but not good effect,and the other 3 monomeric molecules without good inhibition effect on SA were screened.MBC experiment verified that Xanthohumol had good bactericidal effect on SA,and the best bactericidal concen-tration was 12.5 μg·mL-1.After transcriptome sequencing,a total of 63 significantly differentially expressed genes were detec-ted in the group with Xanthohumol added compared to the group without Xanthohumol,including 10 upregulated genes and 53 downregulated genes.Most of the genes were related to ribosomal protein synthesis.Conclusion Xanthohumol had a good bacte-ricidal and bacteriostatic effect on SA.Through transcriptome sequencing,it was found that Xanthohumol might inhibit the bio-synthesis of ribosomes,thus hindering the protein synthesis process of SA and leading to SA death.
国家科技期刊平台
NSTL
万方数据
