三七总皂苷对急性髓系白血病细胞增殖、凋亡的影响
Effect of total saponins of Panax notoginseng on proliferation and apoptosis of KGIa cells in acute myeloid leukemia
黄荔丰 1刘慧丽 1蔡阳艳 1王国伟2
作者信息
- 1. 漳州卫生职业学院转化医学检测应用技术协同创新中心 福建漳州 363000
- 2. 漳州市医院检验科 福建漳州 363000
- 折叠
摘要
目的 观察三七总皂苷对急性髓系白血病细胞株KGIa增殖、凋亡的影响.方法 采用CCK-8实验检测不同浓度的三七总皂苷处理24、48、72 h后对KGIa细胞存活率的影响,倒置显微镜及荧光显微镜下观察KGIa细胞经三七总皂苷作用72 h后细胞形态及凋亡情况的影响,流式细胞术检测KGIa细胞凋亡和细胞周期的变化.结果 三七总皂苷明显抑制KGIa细胞的增殖,呈浓度依赖性.三七总皂苷实验组的KGIa细胞凋亡明显增加,且三七总皂苷使细胞周期阻滞在G0-G1期.结论 三七总皂苷可抑制急性髓系白血病细胞的增殖,可能与阻滞细胞周期和诱导调亡有关.
Abstract
Objective To observe the effects of total saponins of Panax notoginseng on proliferation and apoptosis of acute myeloid leukemia cells KGIa.Methods CCK-8 assay was used to detect the effect of different concentrations of Panax notogin-seng saponins on the survival rate of KGIa cells after 24 hours,48 hours and 72 hours of treatment.The morphology and apopto-sis of KGIa cells treated with total saponins of Panax notoginseng for 72 hours were observed under inverted microscope and fluo-rescence microscope.Flow cytometry was used to detect apoptosis and cell cycle changes.Results The total saponins of Panax notoginseng could significantly inhibit cell proliferation in a concentration dependent manner.The apoptosis of KGIa cells in the notoginseng total saponins group was significantly increased,and notoginseng total saponins blocked the cell cycle in G0-G1 phase.Conclusion Total saponins of Panax notoginseng can inhibit the proliferation of acute myeloid leukemia cells KGIa,which may be related to cell cycle retardation and induction of apoptosis.
关键词
三七总皂苷/KGIa细胞/增殖/凋亡/细胞周期Key words
Panax notoginseng saponin/KGIa cell/proliferation/apoptosis/cell cycle引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据