本研究分别比较了在温室不同水分处理条件和田间自然条件下,包括100％被内生真菌感染(E+)种群,未被内生真菌感染(E-)种群和50％带菌率的E+和E-植株混合种群(M)的3种不同多年生黑麦草(Lolium perenne)种群的生长情况,以分析内生真菌对其寄主多年生黑麦草生长的影响.在模拟田间最大持水量(Field Water Content,FWC)的15％(FWC15)、30％ (FWC30)、45％(FWC45)和60％(FWC60)的4种不同水分处理的温室盆栽试验中,随着土壤水分含量的增大,E+、E-和M种群的株高、分蘖数、株高生长速率、分蘖生长速率、叶宽、叶长、地上组织含水量和地上生物量先逐渐增大,在FWC45水分条件下达到了最大,然后在FWC60水分条件下各项生长指标降低,均低于FWC45水分处理,说明FWC45水分条件最适宜黑麦草幼苗的生长,而FWCl5、FWC30和FWC60水分条件对黑麦草幼苗的生长具有抑制作用;在盆栽各水分条件下,E+种群的分蘖数、株高生长速率、分蘖生长速率、叶宽、地上部组织含水量均显著高于E-和M种群(P＜0.05);E+种群的叶长显著大于E-种群(P＜0.05);在FWC60水分条件下,E+和M种群的地上部生物量差异不显著(P＞0.05),但均显著高于E-种群(P＜0.05),而在其余3个水分条件下,E+种群均显著大于E-和M种群(P＜0.05),但是E-和M种群之间无差异.在田间自然条件下,E+种群的株高、分蘖数、株高生长速率、分蘖生长速率、叶宽、地上部生物量均显著高于E-和M种群(P＜0.05);而E+种群的叶长和地上部组织含水量与M种群差异不显著(P＞0.05),但显著高于E-种群(P＜0.05).这说明内生真菌在温室和田间均能促进多年生黑麦草的生长,并增强其抗旱性和对该地区的环境适应性,内生真菌带菌率越高,其优势越明显.
Effects of fungal endophytes on the growth of perennial ryegrass under drought condition
The present study explored the effects of fungal endophytes on host growth with endophyte-infected (E+),endophyte-free (E-) and mixture of E+ and E-(M) populations of L.perenne under two different growth environmental conditions including greenhouse and field.In green house,four different water treatments including 15 ％ (FWC15),30 ％ (FWC30),45 ％ (FWC45) and 60 ％ (FWC60) of field water content (FWC)were simulated in pots.The results showed that the plant height and tiller numbers,growth rates of plant height and tiller,blade width and length,aboveground water content and aboveground biomass of E+,E-and M gradually increased with the increase of soil moisture content in greenhouse which reached maximum under FWC45 treatment.However,the growth indices of plants under FWC60 moisture condition were less than those of plants under FWC45 treatment which suggested that FWC60 treatment inhibited plants growth.All these results suggested that FWC45 was optimal condition for perennial ryegrass.The tiller numbers,growth rate of plant height and tiller,blade width,aboveground water content of E + populations were significantly higher (P＜0.05) than those of E-and M populations and the blade length of E + populations were significantly higher (P＜0.05) than that of E-populations under each moisture condition in pot experiment.There were no significant difference between E+ and M population for aboveground biomass with FWC60 treatment,while E+ populations were significantly higher (P＜0.05) than that of M populations (P＜0.05) under other treatments.With FWC15,FWC30 and FWC45 treatment,aboveground biomass of E+ population were significantly higher (P＜0.05) than those of E-and M populations although there were no significant difference between E-and M populations,however,aboveground biomass of E+ and M population were significantly higher (P＜0.05) than that of E-populations.Under natural field conditions,the plant height and tiller numbers,growth rates of plant height and tiller,blade width and aboveground biomass of E + populations were significantly higher (P＜0.05) than those of E-and M populations,while the blade length and aboveground water content had no significant difference with M population.The growth indices of E-were significantly(P ＜0.05) less than those of E-population.These results suggested that the fungal endophytes could promote the growth and enhance the drought resistance of L.perenne in the greenhouse and field conditions.
国家科技期刊平台
NSTL
万方数据
维普
