Effects of high temperature treatments on viability of Epichloё seeds of Elymus dahuricus
In the present study,high temperature treatments (60,70 and 75 ℃)was employed to construct Epichloё-free populations of Elymus dahuricus.The results indicated that high temperature treatments can kill Epichloёendophyte in the seeds.After 75 ℃ for 20 days treatment,the Epichloёendophyte infected rates sig-nificantly dropped from 93.17% to 0% (P<0.01),and the germination rate,germinating potential,germina-tion index,vital index and seeding fresh weight of E.dahuricus increased by 36.37%,73.94%,50.38%, 63.38% and 7.79%,respectively(P<0.05).Meanwhile,high temperature improved hypocotyls and radical growth and the hypocotyls and radical length of the treated seedlings increased by 0.54% and 14.43%.There-fore,high temperature of 75 ℃ treatment was an effective way to construct Epichloё-free populations of E.dahuricus which provided some academic bases and references for establishing Epichloё-free populations of grass.
Epichloёhigh temperature treatmentElymus dahuricus