Analysis of EMS and 60 Co-γmutagenesis of Dianthus spiculifolius seeds
In order to develop new varieties of Dianthus spiculifolius ,we mutagenized seeds using EMS and 60 Co-γ rays.Treatment of seeds for 16 h with 1% EMS,close to the half-lethal dose,resulted in a mutation rate of 7.0%.Seeds treated with 300 Gy 60 Co-γrays (dose rate 0.1 Gy·min-1 ,close to the half-lethal dose,re-sulted in a mutation rate of 4.2%.We obtained 1038 phenotypic mutants in the M1 generation,of which 95 were derived from seeds treated with EMS,and 943 were obtained following irradiation of the seeds.This study has enriched the germplasm resources of D .spiculifolius and has provided a technical reference for the muta-genesis of other Dianthus species.
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