Effect of seed soaking with E pichlo?fermentation broth on germination characteristics in perennial ryegrass under drought stress
To explore the in vitro effect of Epichlo?on the drought resistance in perennial ryegrass seeds during germination,the seeds were soaked in three species of Epichlo?(E.gansuensis,E.bromicola,and Epichlo? sp.)fermentation broth,to test the germination index of seeds under different concentrations of polyethylene glycol (PEG)through the paper germination test.The results showed that the seed germination rate,germina-tion force,germination index,vigor index,seeding length,and seedling moisture content were decreased,the mildew rate was increased first and then decreased when the PEG concentration increased,and the root length of seedlings had a peak value at -0.6 MPa.The seeds inactivated and could not germinate when the concentra-tion of PEG was -1.2 MPa.Compared to the control,soaking in Epichlo?fermentation broth significantly(P<0.05)promoted seed germination,seedling growth,and root growth,and effectively reduced the mildew rate (P<0.05).Compared to seed soaking with Achnatherum inebrians Epichlo?fermentation broth,the seed germination index,vigor index,seedling water content,seedling length,and root length of perennial ryegrass were greater than those in the Elymus dahuricus and Festuca sinensis experiments.In short,the fermentation broth of Epichlo?had a certain effect on the germination of perennial ryegrass seeds under drought stress,and the effect of the A.inebrians fermentation broth was the strongest of the three different kinds of fermentation broth.
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