In order to fully utilize the resources of the plant growth promoting rhizobacteria associated with wild forages in Guizhou Province, the siderophore-producing bacterial strain TSQA26-3 was isolated from the rhizosphere soil of wild Trifolium repens and used as the research object in this study. Morphological observation, VITEK-2, and 16S rRNA gene sequence analysis were used to identified the strain. The growth-promoting characteristics of the strain were investigated, and the suitable fermentation medium for the siderophore produced by the strain was optimized using single factor and orthogonal experiments. The result showed that the strain TSQA26-3 was identified as Pseudomonas putida and had the ability to dissolve inorganic phosphorus, produce IAA, and fix nitrogen. Mannitol-Glutamine was proven to be the optimal medium for siderophore production. The fermentation medium formula and cultivation conditions were as follows: 25.0 g·L−1 Mannitol, 2.5 g·L−1 L-Glutamine, 1.0 g·L−1 K2HPO4, 0.25 g·L−1 MgSO4·7H2O, initial pH 7.0, 170 r·min−1, temperature 30 ℃, and medium volume 70 mL in 250 mL conical flask.
关键词
白三叶/植物根际促生菌/铁载体/鉴定/促生特性/培养条件优化/发酵培养基
Key words
Trifolium repens/plant growth promoting rhizobacteria/siderophores/identification/growth-promoting characteristics/optimum fermentation condition/fermentation medium
维普
万方数据