首页|1株白三叶根际产铁载体菌的功能特性及培养条件

1株白三叶根际产铁载体菌的功能特性及培养条件

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为充分利用贵州岩溶山区牧草根际促生菌资源，以野生白三叶(Trifolium repens)根际铁载体产生菌TSQA26-3为研究对象，结合形态观察、VITEK-2全自动微生物鉴定系统和16S rRNA基因序列分析对菌株进行分类鉴定，开展菌株促生特性研究，筛选适宜菌株合成铁载体的发酵基础培养基，通过单因素试验和正交试验相结合，确定最佳培养基成分和培养条件，促进菌株合成铁载体能力的提升。结果表明，菌株TSQA26-3为恶臭假单胞菌(Pseudomonas putida);菌株同时还具有溶解无机磷、产植物生长素(IAA)和固氮能力;优化后的培养基(MG培养基)配方为，甘露醇25。0 g·L−1，L-谷氨酰胺2。5 g·L−1，K2HPO41。0 g·L−1，MgSO4·7H2O 0。25 g·L−1;最佳培养条件为，培养基初始pH 7。0，摇床转速170 r·min−1，培养温度30℃，培养基装液量为每250 mL瓶中装70 mL。
Functional characteristics and optimum fermentation condition of a siderophore-producing bacteria from the rhizosphere of Trifolium repens
In order to fully utilize the resources of the plant growth promoting rhizobacteria associated with wild forages in Guizhou Province, the siderophore-producing bacterial strain TSQA26-3 was isolated from the rhizosphere soil of wild Trifolium repens and used as the research object in this study. Morphological observation, VITEK-2, and 16S rRNA gene sequence analysis were used to identified the strain. The growth-promoting characteristics of the strain were investigated, and the suitable fermentation medium for the siderophore produced by the strain was optimized using single factor and orthogonal experiments. The result showed that the strain TSQA26-3 was identified as Pseudomonas putida and had the ability to dissolve inorganic phosphorus, produce IAA, and fix nitrogen. Mannitol-Glutamine was proven to be the optimal medium for siderophore production. The fermentation medium formula and cultivation conditions were as follows: 25.0 g·L−1 Mannitol, 2.5 g·L−1 L-Glutamine, 1.0 g·L−1 K2HPO4, 0.25 g·L−1 MgSO4·7H2O, initial pH 7.0, 170 r·min−1, temperature 30 ℃, and medium volume 70 mL in 250 mL conical flask.

Trifolium repensplant growth promoting rhizobacteriasiderophoresidentificationgrowth-promoting characteristicsoptimum fermentation conditionfermentation medium

韦鑫、韦兴迪、曾庆飞、李亚娇、丁磊磊、王小利

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贵州省草业研究所,贵州贵阳 550006

白三叶 植物根际促生菌 铁载体 鉴定 促生特性 培养条件优化 发酵培养基

贵州省农业科技支撑计划贵州省科技计划贵州省科研机构创新能力建设专项贵州省草业研究所科技创新课题

黔科合支撑[2023]一般070黔科合基础[2020]1Y062黔科合服企[2022]004草业所科技创新课题[2018]03号

2024

10.11829/j.issn.1001-0629.2023-0305

草业科学
中国草原学会 兰州大学草地农业科技学院

草业科学

CSTPCD北大核心
影响因子：0.854
ISSN：1001-0629
年,卷(期)：2024.41(4)