Genetic diversity of native Elymus sibiricus populations in the Southeastern Margin of Qinghai-Tibetan Plateau as detected by SRAP and SSR markers
The objectives of the study were to quantify the genetic variability in eight natural populations of Elymus sibiricus collected from Southeastern Margin of Qinghai - Tibetan Plateau, and to explore its genetic variation distribution pattern. Sequence-related amplified polymorphism (SRAP) markers and simple sequence repeat (SSR) markers were employed. The follow results were obtained: 1) A total of 384 fragments were i-dentified with 16 SRAP primers sets, of which 86. 98% were polymorphic. Meanwhile, a total of 221 alleles were detected at 16 SSR loci, with 192 (86. 88%) being polymorphic, indicating considerable genetic variation at the species level. 2) The mean gene diversity (He) was estimated to be 0.109 2 and 0. 129 6 within populations detected by SRAP and SSR markers respectively, and 0. 243 4 and 0. 373 2 at the species level. 3) A high level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis both in SRAP (Gst =0. 552 5) and SSR (Gst =0. 515 8) markers, and an indirect estimate of the number of migrants per generation (0. 405 0 by SRAP markers, 0. 469 4 by SSR markers) showed that gene flow was low among populations. 4) Shannon's index analysis and AMOVA analysis displayed the same result that mainly genetic variation of E. sibiricus existed among the populations. In addition, a geographical pattern of population differentiation, where the populations from south, north and middle area of sampling sites were clearly separated from each other, was revealed by cluster analysis. Based on the genetic information available for the native E. sibiricus, some conservation strategies were proposed.
国家科技期刊平台
NSTL
万方数据
维普
