Optimization of the extracting process for alkaloids from Dicranostigma leptopodum by response surface analysis and study on its antibacterial activity in vitro
In this paper, the Dicranostigma leptopodum was researched, on the basis of single factor level de-termination, the optimal extraction conditions were obtained by using the response surface analysis, and the concentrated liquid of extract response surface analysis was measured by antibacterial activity, moreover, the transmission electron microscopy images of Escherichia colt of treated with concentrated liquid of extract from D. leptopodum was discussed. The experiment results demonstrated that the optimum conditions were liquid to solid ratio was 15 mL/g, time of ultrasound was 35 min, refluxing time was 2. 5 h, alcohol was §5%, and the content of alkaloid from D. leptopodum was 9. 33% in the optimum extraction process condition; the optimal extraction conditions were obtained by using the response surface analysis of E. coli, Candida albicans and Pseudomonas aeruginosa of minimal inhibitory concentration and minimal bactericidal concentration were 0. 20, 0. 15, 0. 35 mg/mL and 0. 15, 0. 10, 0. 30 mg/mL. They were observed under transmission electron microscopy to everest to E. coli antibacterial activity effect significantly remarkable. The results also showed that the response surface analysis to D. leptopodum spend alkaloid extraction process optimization are reasonable.
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