首页|塔克拉玛干沙漠生物结皮中几种藻类的系统发育分析

塔克拉玛干沙漠生物结皮中几种藻类的系统发育分析

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新疆塔克拉玛干沙漠中富含多种沙漠藻类,严酷的沙漠环境造就了这些沙漠微藻独特的生物学特性,但对于这些沙漠微藻的研究,相关报道较少。本研究以新疆塔克拉玛干沙漠沙样为素材,通过实验室培养,以有限稀释法和平板法进行分离,分离得到6株沙漠微藻。形态学观察后初步鉴定5株属于绿藻门(TLD2A1,TLD2B, TLD7A-2,TLD6B,TLD7B-5),1株为蓝藻门(TLD5A1)。分别比对分析了5株绿藻的18S rDNA和其中3株的5.8S rDNA-ITS序列,1株蓝藻 TLD5A1的16S rDNA 序列,并进行了系统发育分析。结果表明,TLD2A1和TLD6 B与小球藻科物种亲缘关系很近,TLD2 B和衣藻科物种亲缘关系很近。TLD7 B-5形态呈新月形,分子鉴定与环藻科物种亲缘关系较近,TLD5 A1与念珠藻科的物种聚为一支,同时与念珠藻属的椭孢念珠藻同源性高达97%。本研究确定了6株沙漠微藻的分类地位,为后续塔克拉玛干沙漠微藻的研究工作提供理论依据。
Phylogenetic analyses on the biological crusts of several algae in the Taklimakan Desert
The unique biological property of desert algae comes from the extreme environment of the Taklima-kan Desert in Xinjiang,but so far there are no related reports on the desert algae.To study the microalgae spe-cies of the Takilimakan Desert,6 desert microalgae were isolated from sand samples using 9 6-well plate limited dilution and spread plate methods.Morphologically,5 of the strains investigated were Chlorophyta (TLD2A1, TLD2B,TLD6B,TLD7A-2,TLD7B-5)while TLD5A1 was a Cyanobacterium.TLD2A1 and TLD2B showed a close relationship with Chlorellaceae,TLD2B and TLD7A-2 with Chlamydomonadaceae,and TLD7B-5 with Tetranephris brasiliensis of the Sphaeropleales.TLD5A1 and Nostoc clustered in a single clade,and the TLD5A1 strain was 97% similar to Nostocellipsosporum.This study determined the taxonomic status of the 6 strains of microalgae in the desert,providing a theoretical basis for future research of the Taklimakan Desert al-gae.

Taklimakan desertChlorophytaCyanobacteriaphylogeny

王丹、龚春霞、苟亚峰、周路、朱军保、高剑峰

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石河子大学生命科学学院,新疆 石河子 832000

塔克拉玛干沙漠 绿藻 蓝藻 系统发育

国家重点基础研究发展规划项目(973计划)

2011-CB200900-G

2014

草业学报
中国草原学会 兰州大学草地农业科技学院

草业学报

CSTPCDCSCD北大核心
影响因子:4.082
ISSN:1004-5759
年,卷(期):2014.(3)
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