Tissue culture in vitro and plant regeneration ofViola prionantha
The effects of various hormone ratios on adventitious buds,callus inductions and differentiation were investigated using the cotyledon node,leaf blade and petiole of Viola prionantha as explants.Finally,a plant regeneration system via tissue culture was established.The results showed that the optimal medium for adven-titious bud induction from the cotyledon node and petiole was MS+2.0 mg/L 6-BA+0.1 mg/L NAA,but the leaf blade was not suitable as an explant.Three kinds of explants could induce callus formation.The optimal medium for callus induction from the cotyledon node and petiole was MS+1.0 mg/L 6-BA+0.5 mg/L 2,4-D with the highest callus induction rate being 98.3% and 96.7%,respectively.The optimal medium for callus induction from the leaf blade was MS+1.0 mg/L 6-BA+0.5 mg/L 2,4-D+0.05 mg/L NAA with the highest callus induction rate being 88.3%.The best medium for callus differentiation was MS+1.5 mg/L 6-BA+0.1 mg/L NAA with the highest callus differentiation rate being 100%.The optimal medium for the proliferation of adventitious buds was MS+1.5 mg/L 6-BA+0.075 mg/L NAA,the highest proliferation times being 4.68. The rooting rate of regenerated plants was up to 92.3% on half strength MS medium supplemented with 1.0 mg/L 6-BA.Rooted plantlets were grown on mixture with perlite:sand:humus (1:2:2);survival rate was up to 100% with good growth.
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