摘要
目的:探讨RNA结合蛋白ZCCHC17对肝细胞癌增殖、迁移、侵袭能力的影响.方法:采用RT-qPCR和Western blot检测所选择不同细胞株ZCCHC17的表达水平;克隆形成实验、CCK-8实验、划痕实验及Transwell侵袭实验检测细胞株增殖、迁移、侵袭能力.结果:ZCCHC17在人正常肝细胞株L02中低表达(P＞0.05);在肝细胞癌Bel7404细胞株中高表达(P＜0.05),在肝细胞癌MHCC97H细胞株中低表达(P＞0.05).克隆形成实验及CCK-8实验结果显示,敲低ZCCHC17后Bel7404细胞株的增殖能力减弱(P＜0.05);划痕实验结果显示,敲低ZCCHC17后Bel7404细胞株的迁移能力减弱(P＜0.05);Tran-swell 实验结果显示,敲低ZCCHC17后Bel7404细胞株的侵袭能力减弱(P＜0.05).结论:ZCCHC17高表达能促进肝细胞癌的增殖、迁移、侵袭能力.
Abstract
Objective:To explore the effect of RNA binding ZCCHC17 on proliferation,migration and invasion ability of hepato-cellular carcinoma(HCC).Methods:RT-qPCR and Western blot were used to detect the expression levels of the cell lines ZCCHC17 selected.Then,the proliferation,migration,and invasion ability of cell lines were examined by clonformation assay,CCK-8 assay,scratch assay and Transwell invasion assay.Results:ZCCHC 17 was lowly expressed in human normal liver cell line L02(P＞0.05),high ex-pression was observed in the Bel7404 hepatocellular carcinoma cell line(P＜0.05),and low expression was observed in the MH-CC97H hepatocellular carcinoma cell line was diminished after ZCCHC17 knockdown(P＞0.05).The results of clone formation assay and CCK-8 showed that the proliferation of Bel7404 cell lines was diminished after ZCCHC17 knockdown(P＜0.05),the scratch test results showed that the migration ability of Bel7404 cell lines decreased(P＜0.05),the Transwell experiment showed that the invasion ability of Bel7404 cell lines was weakened(P＜0.05).Conclusion:High expression of ZCCHC17 can promote the proliferation,migra-tion and invasion of hepatocellular carcinoma.
维普
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